TOXICS COMPARISON OF GRAPHENE NANO SHEETS AND CARBON NANOTUBES EXPLORING HEPATO-INTESTINAL AND HEMATOLOGICAL DISPARITIES IN CIRRHINUS MRIGALA

Abstract

This study was conducted to explore the comparative toxicity exploration of graphenenano sheets and carbon nanotubes in fish Cirrhinusmrigala. The experiment was performed in Department of Zoology, University of Education, Lahore, Jauharabad campus. The experimental fish were obtained from chashma fish hatchary and transported to experimental site. The experiment was started after the division of fish in seven different groups (n=70) named as C, GNS-I, GNS-II, GNS-III, CNT-I, CNT-II and CNT-III. Group C was control group while group GNS-I, GNS-II and GNS-III were treated waterborne with 40.45, 60.58 and 121.37 mg/L of graphenenano tubes. Group CNT-I, CNT-II and CNT-III were treated orally with 10.92, 16.38 and 32.76 mg/kg of carbon nano tubes. The hematological results reveal that hemoglobin, erythrocytes count, pack cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration and lymphocytes were significantly (p<0.05) decreased while mean corpuscular volume, leucocyte count, neutrophils and monocytes were significantly increased in both graphenenanosheets and carbon nanotubes exposed fish.The liver was reported with histological abnormalities such as Pyknotic nuclei, Atrophy, Acute cellular swelling, melanomacrophage centers, hepatic vacuolation, sinusoid dilation, hydropic degeneration, necrosis and perivascular degeneration. In the intestine intestinal damage, collapsed epithelial layer, intestinal interior architecture damage, atrophy, bigger intercellular gaps, and degenerated goblet cells were reported. This study concludes that graphenenano sheets and carbon nano tubes are toxic at different doses in correlation to the route of exposure.

Keywords: Graphenenano sheets, Carbon nanotubes, Fish Cirrhinusmrigala, Toxicity exploration, Hematological results