ASSESSMENT OF LC50 INCLUDING HEPATO-INTESTINAL ABNORMALITIES IN CIRRHINUS MRIGALA INDUCED WITH GRAPHENE NANO SHEETS

Abstract

This study investigates the hepato-intestinal abnormalities induced with graphenenano sheets in Cirrhinus mrigala.The study was initially started with the aim to investigating the LC50 of GNS by the formation of groups and named as C, GNS-1, GNS-II, GNS-III, GNS-IV, GNS-V and GNS-VI.The fish (n=70) in these groups were administrated with 0, 250, 500, 750, 1000, 1250, and 1500 mg/L of CNS in water for 96-hrs. The mortality within 96-hrs was recorded as 0, 0, 1, 2, 3, 5 and 7 in Nos. The LC50 calculated in this study was 121.37 mg/L after applying probit analysis. In this study various histological changes in intestine are necrosis in the villi of the intestine, destroyed intestinal integrity, inflammation in the intestine, disintegration of the epithelial layer, vacoulation and degenerated goblet cell and liver was reported with histological alterations such as central vein injury, melanomacrophage center, pyknotic nuclei, dilation of sinusoids, vacuolation, necrosis of hepatocytes and congestion. In conclusion, this study reveals significant hepato-intestinal abnormalities in Cirrhinus mrigala induced by graphenenano sheets, with observed mortality patterns and histological changes, underscoring the potential impact of graphene exposure on the health of aquatic organisms.

Keywords: Hepato-intestinal abnormalities, Graphenenano sheets, Cirrhinus mrigala, LC₅₀, Mortality, Histological changes